It will hurt their prospects of making the Champions League cut after a shocking loss.
The Old Lady is facing a season without the Champions League in almost 11 years, as they got their points docked for the second time this season. Juventus was demoted from second place in Serie A to seventh as a result of the most recent penalty for incorrect accounting in the team's transfer operations.
Juventus is five points behind AC Milan as they move to the 4th spot in the Serie A rankings.
"What was established by the fifth instance of judgment in this matter, which began more than a year ago, arouses great bitterness in the club and in its millions of supporters who, in the absence of clear rules, find themselves extremely penalized with the application of sanctions that do not seem to take into account the principle of proportionality," Juventus added on Twitter.
"While not ignoring the need for urgency, which Juventus has never shied away from during the proceedings, it is emphasized that these are facts that still have to be evaluated by a judge."
The Italian Olympic Committee's top sports court accepted the appeal from Juventus last month, suspending the points reduction. It also remanded the case for further consideration to the football federation's appeals court.